Methyl Green is a nuclear counterstain that stains cell nuclei light green. The Bioenno Methyl Green Solution can be applied to routine histological sections, sections that histochemistry, immunohistochemistry, in situ hybridization, or Golgi staining has been finished, as well as cultured cells. The Bioenno formulated solution has been purified with chloroform and proven to generate excellent nuclei staining on immunohistochemistry sections developed with 3,3’-diaminobenzidine (DAB) or benzidine dihydrochloride (BDHC). The staining can be carried out at room temperature (18-25ºC) and generally takes 1-5 minutes. Differentiation is not necessary in most cases. This counterstain solution is suitable for use with non-aqueous mountants.