MiniOne Eletrophoresis 101 Mini Lab - Who is your Daddy Lab Due 11/4/2024

Ideal as a hands-on introductory lab to electrophoresis. With this MiniLab, students cast their own gels and run real DNA. Results can be visualized within a 45 minute class period, eliminating need for multiple sessions. Students also learn how to do a semi-log plot of known vs unknown samples.

• Give your students the complete hands-on experience of casting, loading, running, documenting, and analyzing electrophoresis gels.

• Students will develop an understanding of gel electrophoresis principles by observing separation of dye and DNA samples

• Students use their data to create a standard curve for DNA fragments of known molecular weight the calculate the weight of unknown DNA fragments

• An essential introduction to electrophoresis and one of our most popular labs

• Store in the refrigerator; GreenGel:tm: Cups should be left in the original package and protected from light; guaranteed stable for six months with proper storage

• Ten 1% agarose GreenGel:tm: Cups

• Three color dye samples and three DNA samples

• One bottle of 100 mL Tris-Borate-EDTA (TBE) buffer concentrate, enough to make 2L of 1X running buffer

• One bag of 0.65 mL microcentrifuge tubes

• One bag of 2 – 200 µL micropipette tips

• Teacher’s guide

Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.

Materials include: